cdna (Illumina Inc)
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Illumina Inc
cdna
Cdna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 11886 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+library+preparation/Nextera+XT+DNA+Library+Preparation+Kit/pm41826404-319-16-10
Average 99 stars, based on 11886 article reviews
Cdna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 11886 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+library+preparation/Nextera+XT+DNA+Library+Preparation+Kit/pm41826404-319-16-10
Average 99 stars, based on 11886 article reviews
cdna - by Bioz Stars,
2026-10
99/100 stars
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cDNA Library Assay:Article Title: Claudin-17 Deficiency Drives Vascular Permeability and Inflammation Causing Lung Injury Article Snippet: RNA quality was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), ensuring an RNA Integrity Number (RIN) greater than 5. .. Total RNA was processed for Article Title: Single-cell analysis of 5-aminolevulinic acid intraoperative labeling specificity for glioblastoma Article Snippet: Beads from each region were extracted separately, washed sequentially with TE/SDS buffer (10 mM Tris–HCl, 1 mM EDTA, 0.5% SDS), TE/TW buffer, and nuclease-free water. cDNA amplification was performed in 50 μL PCR solution [1X Hifi Hot Start Ready mix (Kapa Biosystems, cat# KK2601), 1 μM SMRTpcr primer (Supplementary Table S6)], with 16 amplification cycles (95 °C 3 min, 4 cycles of (98 °C 20 s, 65 °C 45 s, 72 °C 3 min), 12 cycles of (98 °C 20 s, 67 °C 20 s, 72 °C 3 min), 72 °C 5 min). .. PCR product was purified using SPRI paramagnetic bead (Beckman, cat# A63881) with a bead-to-sample volume ratio of 0.6:1. Article Title: Influence of Epigenetic Regulators Expression on Gastric Cancer Prognosis Article Snippet: .. RNA was extracted, quality checked, and used for Article Title: Transcriptional Adaptations to Muscle Loading in a Murine Model of Achilles Tendinopathy Article Snippet: .. Samples of adequate quality (RNA integrity number (RIN) ≥ 4.0; Agilent 2100) but low RNA concentration were combined with other samples from the same treatment group to achieve the minimum total RNA required (100 ng), resulting in n < 6 in some groups (Table ). Article Title: Modeling Masticatory Myalgia to Headache-like Referred Pain Triggers Local Gene Plasticity at Referred Pain Sites Article Snippet: Frozen and sliced MM as previously described [ ] and freshly dissected entire dura matter were homogenized in Rn-easy solution (Qiagen) using a Bead Mill Homogenizer (Omni International, Kennesaw, GA). .. Extraction of RNA, RNA quality and integrity control, Article Title: Claudin-17 Deficiency Drives Vascular Permeability and Inflammation Causing Lung Injury. Article Snippet: RNA quality was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), ensuring an RNA Integrity Number (RIN) greater than 5. .. Total RNA was processed for Article Title: Article Snippet: .. 20 21 cDNA library construction and sequencing 22 Total RNA Globin reduction and Article Title: Identification of Rcr12 , a single dominant clubroot resistance gene near Rcr6 on chromosome B3 of Brassica nigra Article Snippet: The Experion RNA StdSens analysis kit (Bio-Rad; Montreal, Canada) was employed to ensure RNA quality, with a stipulation that the RNA integrity number should exceed 8 for each sample. .. Subsequent steps in the process included Polymerase Chain Reaction:Article Title: Single-cell analysis of 5-aminolevulinic acid intraoperative labeling specificity for glioblastoma Article Snippet: Beads from each region were extracted separately, washed sequentially with TE/SDS buffer (10 mM Tris–HCl, 1 mM EDTA, 0.5% SDS), TE/TW buffer, and nuclease-free water. cDNA amplification was performed in 50 μL PCR solution [1X Hifi Hot Start Ready mix (Kapa Biosystems, cat# KK2601), 1 μM SMRTpcr primer (Supplementary Table S6)], with 16 amplification cycles (95 °C 3 min, 4 cycles of (98 °C 20 s, 65 °C 45 s, 72 °C 3 min), 12 cycles of (98 °C 20 s, 67 °C 20 s, 72 °C 3 min), 72 °C 5 min). .. PCR product was purified using SPRI paramagnetic bead (Beckman, cat# A63881) with a bead-to-sample volume ratio of 0.6:1. Purification:Article Title: Single-cell analysis of 5-aminolevulinic acid intraoperative labeling specificity for glioblastoma Article Snippet: Beads from each region were extracted separately, washed sequentially with TE/SDS buffer (10 mM Tris–HCl, 1 mM EDTA, 0.5% SDS), TE/TW buffer, and nuclease-free water. cDNA amplification was performed in 50 μL PCR solution [1X Hifi Hot Start Ready mix (Kapa Biosystems, cat# KK2601), 1 μM SMRTpcr primer (Supplementary Table S6)], with 16 amplification cycles (95 °C 3 min, 4 cycles of (98 °C 20 s, 65 °C 45 s, 72 °C 3 min), 12 cycles of (98 °C 20 s, 67 °C 20 s, 72 °C 3 min), 72 °C 5 min). .. PCR product was purified using SPRI paramagnetic bead (Beckman, cat# A63881) with a bead-to-sample volume ratio of 0.6:1. Concentration Assay:Article Title: Transcriptional Adaptations to Muscle Loading in a Murine Model of Achilles Tendinopathy Article Snippet: .. Samples of adequate quality (RNA integrity number (RIN) ≥ 4.0; Agilent 2100) but low RNA concentration were combined with other samples from the same treatment group to achieve the minimum total RNA required (100 ng), resulting in n < 6 in some groups (Table ). DNA Library Preparation:Article Title: Transcriptional Adaptations to Muscle Loading in a Murine Model of Achilles Tendinopathy Article Snippet: .. Samples of adequate quality (RNA integrity number (RIN) ≥ 4.0; Agilent 2100) but low RNA concentration were combined with other samples from the same treatment group to achieve the minimum total RNA required (100 ng), resulting in n < 6 in some groups (Table ). Sequencing:Article Title: Transcriptional Adaptations to Muscle Loading in a Murine Model of Achilles Tendinopathy Article Snippet: .. Samples of adequate quality (RNA integrity number (RIN) ≥ 4.0; Agilent 2100) but low RNA concentration were combined with other samples from the same treatment group to achieve the minimum total RNA required (100 ng), resulting in n < 6 in some groups (Table ). Article Title: Modeling Masticatory Myalgia to Headache-like Referred Pain Triggers Local Gene Plasticity at Referred Pain Sites Article Snippet: Frozen and sliced MM as previously described [ ] and freshly dissected entire dura matter were homogenized in Rn-easy solution (Qiagen) using a Bead Mill Homogenizer (Omni International, Kennesaw, GA). .. Extraction of RNA, RNA quality and integrity control, Article Title: Article Snippet: .. 20 21 cDNA library construction and sequencing 22 Total RNA Globin reduction and Extraction:Article Title: Modeling Masticatory Myalgia to Headache-like Referred Pain Triggers Local Gene Plasticity at Referred Pain Sites Article Snippet: Frozen and sliced MM as previously described [ ] and freshly dissected entire dura matter were homogenized in Rn-easy solution (Qiagen) using a Bead Mill Homogenizer (Omni International, Kennesaw, GA). .. Extraction of RNA, RNA quality and integrity control, Control:Article Title: Modeling Masticatory Myalgia to Headache-like Referred Pain Triggers Local Gene Plasticity at Referred Pain Sites Article Snippet: Frozen and sliced MM as previously described [ ] and freshly dissected entire dura matter were homogenized in Rn-easy solution (Qiagen) using a Bead Mill Homogenizer (Omni International, Kennesaw, GA). .. Extraction of RNA, RNA quality and integrity control, Sample Prep:Article Title: Modeling Masticatory Myalgia to Headache-like Referred Pain Triggers Local Gene Plasticity at Referred Pain Sites Article Snippet: Frozen and sliced MM as previously described [ ] and freshly dissected entire dura matter were homogenized in Rn-easy solution (Qiagen) using a Bead Mill Homogenizer (Omni International, Kennesaw, GA). .. Extraction of RNA, RNA quality and integrity control, Article Title: Identification of Rcr12 , a single dominant clubroot resistance gene near Rcr6 on chromosome B3 of Brassica nigra Article Snippet: The Experion RNA StdSens analysis kit (Bio-Rad; Montreal, Canada) was employed to ensure RNA quality, with a stipulation that the RNA integrity number should exceed 8 for each sample. .. Subsequent steps in the process included |